Hotspot

rps15-ycf1

SSC · 121543-121859 · 317 bp

Back to Matsumurella

Pi 0.0126

MarkerSeek score 47.90

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 317
variable_sites: 9
pi: 0.0126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	TGATACCAATAACGGCAGCC	50.0	50.0	58.0	58.0	645 bp	1.0	81.8
rps15-ycf1_p2	CAGCAGTCGTTGACGTTTTC	AGCAGCCAAGTTTCACATGA	50.0	45.0	58.0	58.0	770 bp	1.0	81.7
rps15-ycf1_p3	AGTCGTTGACGTTTTCCCAA	AGCAGCCAAGTTTCACATGA	45.0	45.0	58.0	58.0	766 bp	1.0	81.5
rps15-ycf1_p4	CAGCAGTCGTTGACGTTTTC	GCAGCCAAGTTTCACATGAC	50.0	50.0	58.0	57.9	769 bp	1.0	81.4
rps15-ycf1_p5	CAGCAGTCGTTGACGTTTTC	ACGAAAGCAGAACTCCGTTT	50.0	45.0	58.0	58.1	1322 bp	1.0	80.8