Hotspot

psaJ-rpl33

LSC · 66423-66895 · 473 bp

Back to Matsumurella

Pi 0.00904

MarkerSeek score 53.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 473
variable_sites: 9
pi: 0.00904
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	CTATGGTTCGGGGCTTTAGC	TCCAAAATCACCGTTACCCG	55.0	50.0	58.4	58.2	593-601 bp	1.0	77.5
psaJ-rpl33_p2	GCTATGGTTCGGGGCTTTAG	TCCAAAATCACCGTTACCCG	55.0	50.0	58.4	58.2	594-602 bp	1.0	77.5
psaJ-rpl33_p3	CTATGGTTCGGGGCTTTAGC	CGCATCTTTCCTCTTAGCCA	55.0	50.0	58.4	57.7	573-581 bp	1.0	76.6
psaJ-rpl33_p4	CGTGGCCCCAGTACTAAGTA	TCCAAAATCACCGTTACCCG	55.0	50.0	58.5	58.2	615-623 bp	1.0	76.5
psaJ-rpl33_p5	TGGCCCCAGTACTAAGTACG	TCCAAAATCACCGTTACCCG	55.0	50.0	58.5	58.2	613-621 bp	1.0	76.5