Hotspot

ndhC-trnV-UAC

LSC · 49821-50785 · 965 bp

Back to Matsumurella

Pi 0.00663

MarkerSeek score 64.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 13
pi: 0.00663
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGGGCTAAAATCCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1065-1096 bp	1.0	81.0
ndhC-trnV-UAC_p2	TTCTCCGGCCCTTTCCTAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	1086-1117 bp	1.0	78.3
ndhC-trnV-UAC_p3	GGGGCTAAAATCCCGGAAAT	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.9	57.5	1064-1095 bp	1.0	78.3
ndhC-trnV-UAC_p4	GGGGCTAAAATCCCGGAAAT	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1053-1084 bp	1.0	77.1
ndhC-trnV-UAC_p5	CCCTTTCCTAATCGGGGCTA	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.6	57.8	1078-1109 bp	1.0	77.1