Hotspot

trnF-GAA-ndhJ

LSC · 47487-48149 · 663 bp

Back to Matsumurella

Pi 0.00639

MarkerSeek score 57.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 663
variable_sites: 8
pi: 0.00639
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	355-805 bp	1.0	84.2
trnF-GAA-ndhJ_p2	CTACATTGGGAATGGTCGGG	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	372-822 bp	1.0	83.9
trnF-GAA-ndhJ_p3	TGCTACATTGGGAATGGTCG	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.0	58.1	374-824 bp	1.0	83.9
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	319-769 bp	1.0	83.8
trnF-GAA-ndhJ_p5	GGGATAGCTCAGCTGGTAGA	GATAATCATCCGCGCCTGAA	55.0	50.0	58.0	58.2	394-844 bp	1.0	83.2