Hotspot

trnT-UGU-trnL-UAA

LSC · 45877-46548 · 672 bp

Back to Matsumurella

Pi 0.00863

MarkerSeek score 63.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 672
variable_sites: 11
pi: 0.00863
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	GGTTTCCCACACTAAGGCTC	55.0	55.0	57.9	58.2	772-778 bp	1.0	78.7
trnT-UGU-trnL-UAA_p2	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	733-739 bp	1.0	78.1
trnT-UGU-trnL-UAA_p3	TAGGAAAAAGCCGGCTATCG	GGTTTCCCACACTAAGGCTC	50.0	55.0	57.8	58.2	823-829 bp	1.0	77.8
trnT-UGU-trnL-UAA_p4	GGAATCGAACCGATGACCAT	GGTTTCCCACACTAAGGCTC	50.0	55.0	57.8	58.2	804-810 bp	1.0	77.7
trnT-UGU-trnL-UAA_p5	TAGGAAAAAGCCGGCTATCG	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	784-790 bp	1.0	77.3