Hotspot

trnK-UUU-rps16

LSC · 4195-5015 · 821 bp

Back to Matsumurella

Pi 0.00414
MarkerSeek score 58.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
821
variable_sites
7
pi
0.00414
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 890-1014 bp 1.0 70.2
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAA 50.0 45.0 58.2 56.5 889-1013 bp 1.0 70.2
trnK-UUU-rps16_p3 TCTAGCCGCACTTAAAAGCC AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 904-1028 bp 1.0 69.4
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA AGGAACTTCCGCCTAATCAA 50.0 45.0 58.3 56.5 899-1023 bp 1.0 69.3
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AGGAACTTCCGCCTAATCAAA 50.0 42.9 58.2 57.3 889-1013 bp 1.0 68.2