Hotspot

atpF

LSC · 11470-12980 · 1511 bp

Back to Masdevallia

Pi 0.00942

MarkerSeek score 65.70

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1511
variable_sites: 14
pi: 0.00942
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TTCGGAAGGAAAGGGAAAAA	AGCGGAGTGATACAAAAAGAAC	40.0	40.9	55.1	57.0	1630-1636 bp	1.0	41.9
atpF_p2	TTCGGAAGGAAAGGGAAAAA	GCGGAGTGATACAAAAAGAACT	40.0	40.9	55.1	57.0	1629-1635 bp	1.0	41.9
atpF_p3	TTTCGGAAGGAAAGGGAAAA	AGCGGAGTGATACAAAAAGAAC	40.0	40.9	55.1	57.0	1631-1637 bp	1.0	41.9
atpF_p4	TTTCGGAAGGAAAGGGAAAA	GCGGAGTGATACAAAAAGAACT	40.0	40.9	55.1	57.0	1630-1636 bp	1.0	41.9
atpF_p5	TTTTCGGAAGGAAAGGGAAA	GCGGAGTGATACAAAAAGAACT	40.0	40.9	55.1	57.0	1631-1637 bp	1.0	41.9