Hotspot

ndhE-ndhG

SSC · 120303-120454 · 152 bp

Back to Masdevallia

Pi 0.0461

MarkerSeek score 65.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 152
variable_sites: 7
pi: 0.0461
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AGTGCTCTAACCATGCTTCG	GTGGGAGCCGTCAATGTATT	50.0	50.0	58.0	58.0	577 bp	1.0	81.2
ndhE-ndhG_p2	AGTGCTCTAACCATGCTTCG	TTGCTTGGTTCAAGAACGGA	50.0	45.0	58.0	58.0	989-990 bp	1.0	80.6
ndhE-ndhG_p3	AGTGCTCTAACCATGCTTCG	TTATGTGGGAGCCGTCAATG	50.0	50.0	58.0	58.0	581 bp	0.5	59.6
ndhE-ndhG_p4	CTCTAACCATGCTTCGGCTT	TTATGTGGGAGCCGTCAATG	50.0	50.0	58.0	58.0	577 bp	0.5	59.5
ndhE-ndhG_p5	CTCTAACCATGCTTCGGCTT	GTGGGAGCCGTCAATGTATT	50.0	50.0	58.0	58.0	573 bp	0.5	59.5