Hotspot

clpP-psbB

LSC · 71923-72767 · 845 bp

Back to Masdevallia

Pi 0.0221

MarkerSeek score 66.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 845
variable_sites: 18
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	1023-1030 bp	1.0	82.9
clpP-psbB_p2	TATACGTCAACCCAAGCTGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	58.0	57.9	1018-1025 bp	1.0	82.8
clpP-psbB_p3	CTTTTGGAACACCAATGGGC	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.8	57.9	969-976 bp	1.0	81.8
clpP-psbB_p4	TTTGGAACACCAATGGGCAT	GGCCCAACCAGAAACTAGAG	45.0	55.0	58.3	57.9	967-974 bp	1.0	80.9
clpP-psbB_p5	TCCAGGACTCCGAAAAGGTA	GGCCCAACCAGAAACTAGAG	50.0	55.0	57.7	57.9	989-996 bp	1.0	80.5