Hotspot

ycf4-cemA

LSC · 59963-60710 · 748 bp

Back to Masdevallia

Pi 0.0269

MarkerSeek score 67.70

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 748
variable_sites: 20
pi: 0.0269
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	ATTCCCTTGACCCGTACTCA	CCACCAGGGCAAAAAGAGTA	50.0	50.0	58.1	57.7	916-921 bp	1.0	79.4
ycf4-cemA_p2	TTCCCTTGACCCGTACTCAT	CCACCAGGGCAAAAAGAGTA	50.0	50.0	58.1	57.7	915-920 bp	1.0	79.4
ycf4-cemA_p3	ACAAAAAGCTGCCGAATTGG	CCACCAGGGCAAAAAGAGTA	45.0	50.0	57.8	57.7	869-874 bp	1.0	78.7
ycf4-cemA_p4	AAAGGGACAAGGAGCCATTC	CCACCAGGGCAAAAAGAGTA	50.0	50.0	57.8	57.7	932-937 bp	1.0	78.0
ycf4-cemA_p5	CATTCCCTTGACCCGTACTC	CCACCAGGGCAAAAAGAGTA	55.0	50.0	57.7	57.7	917-922 bp	1.0	77.3