Hotspot

trnF-GAA-ndhJ

LSC · 47862-48032 · 171 bp

Back to Masdevallia

Pi 0.0307

MarkerSeek score 69.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 171
variable_sites: 5
pi: 0.0307
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	58.1	57.9	260-268 bp	1.0	82.7
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGGCTGGCCTTTACGTAAAG	50.0	50.0	58.1	57.9	261-269 bp	1.0	82.6
trnF-GAA-ndhJ_p3	AGGATGATGCACGGGAAATC	GGCTGGCCTTTACGTAAAGA	50.0	50.0	58.0	57.9	319-327 bp	1.0	82.2
trnF-GAA-ndhJ_p4	AGGATGATGCACGGGAAATC	AGGCTGGCCTTTACGTAAAG	50.0	50.0	58.0	57.9	320-328 bp	1.0	82.1
trnF-GAA-ndhJ_p5	AGGATGATGCACGGGAAATC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.0	57.9	332-340 bp	1.0	81.8