Hotspot

psaA-ycf3

LSC · 41336-41974 · 639 bp

Back to Masdevallia

Pi 0.018

MarkerSeek score 73.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 639
variable_sites: 11
pi: 0.018
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	760-787 bp	1.0	81.5
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	759-786 bp	1.0	81.5
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	794-821 bp	1.0	80.8
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	763-790 bp	1.0	80.3
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	CTTGGTTCGATCAAGCTGCT	50.0	50.0	57.9	58.6	798-825 bp	1.0	78.6