Hotspot

petN-psbM

LSC · 28739-29597 · 859 bp

Back to Masdevallia

Pi 0.0247

MarkerSeek score 70.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 859
variable_sites: 20
pi: 0.0247
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GGGACATCATTCGCATGGAT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.1	58.3	968-992 bp	1.0	82.7
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	ACGTCAAAACAGTCAGCCAA	50.0	45.0	58.2	58.3	947-971 bp	1.0	82.4
petN-psbM_p3	GGGACATCATTCGCATGGAT	GCGCTTATTGCTACTACGCT	50.0	50.0	58.1	58.5	1026-1050 bp	1.0	80.4
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	GCGCTTATTGCTACTACGCT	50.0	50.0	58.2	58.5	1005-1029 bp	1.0	80.0
petN-psbM_p5	GGGACATCATTCGCATGGAT	TACGTCAAAACAGTCAGCCA	50.0	45.0	58.1	57.4	969-993 bp	1.0	79.9