Hotspot

trnS-GCU-trnG-UCC

LSC · 7848-8781 · 934 bp

Back to Masdevallia

Pi 0.0231
MarkerSeek score 63.00
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
934
variable_sites
21
pi
0.0231
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGGAAAGACCCCTTAACTGT 50.0 45.0 58.0 56.0 1039-1062 bp 1.0 67.0
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGGAAAGACCCCTTAACTGT 50.0 45.0 58.0 56.0 1038-1061 bp 1.0 67.0
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA GGAAAGACCCCTTAACTGTTGA 50.0 45.5 58.0 57.9 1038-1061 bp 1.0 66.6
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC GGAAAGACCCCTTAACTGTTGA 50.0 45.5 58.0 57.9 1037-1060 bp 1.0 66.6
trnS-GCU-trnG-UCC_p5 CAATCCGACGCTTTAGTCCA TGGAAAGACCCCTTAACTGTTG 50.0 45.5 58.0 58.2 1039-1062 bp 1.0 65.9