Hotspot

ycf1

Genome · 123349-128136 · 4788 bp

Back to Marsilea

Pi 0.484

MarkerSeek score 74.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 4788
variable_sites: 1899
pi: 0.484
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGGGGTGTTTTTGATCCACT	ACCGGGATTGGATTTTCGAG	50.0	50.0	58.0	58.0	1117 bp	0.5	59.6
ycf1_p2	ACGAACATCACTCTCCATGC	ACCGGGATTGGATTTTCGAG	50.0	50.0	58.0	58.0	779 bp	0.5	59.6
ycf1_p3	CCCTTCACAAGTCTACGACG	ACCGGGATTGGATTTTCGAG	55.0	50.0	58.0	58.0	892 bp	0.5	59.5
ycf1_p4	GGGGATCGATGGAGAAAACC	ACCGGGATTGGATTTTCGAG	55.0	50.0	58.0	58.0	552 bp	0.5	59.4
ycf1_p5	GGGGGTGTTTTTGATCCACT	GGAACTCTTGTGGTGGGTTT	50.0	50.0	58.0	57.9	1386 bp	0.5	59.4