Hotspot

rps15

Genome · 122766-123035 · 270 bp

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Pi 0.473

MarkerSeek score 68.20

Primer available yes

Label rps15

Feature Metadata

label_name: rps15
feature_type: gene
region: Genome
length_bp: 270
variable_sites: 80
pi: 0.473
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.63
markerseek_score: 68.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15_p1	CAACTCCTCCTACGCGAAAA	CTATCACGCGCTTATCTCCC	50.0	55.0	57.9	58.0	1535 bp	0.5	59.3
rps15_p2	CAACTCCTCCTACGCGAAAA	TATCTCCCGCAGTGGTAGAG	50.0	55.0	57.9	58.0	1523 bp	0.5	59.3
rps15_p3	CAACTCCTCCTACGCGAAAA	TCTCCTACTTCCGAGACGTC	50.0	55.0	57.9	58.0	1465 bp	0.5	59.2
rps15_p4	ATCATTCGCATACCGGTAGC	CTATCACGCGCTTATCTCCC	50.0	55.0	57.9	58.0	1506 bp	0.5	59.2
rps15_p5	ATCATTCGCATACCGGTAGC	TATCTCCCGCAGTGGTAGAG	50.0	55.0	57.9	58.0	1494 bp	0.5	59.2