Hotspot

ndhG

Genome · 117812-118405 · 594 bp

Back to Marsilea

Pi 0.482

MarkerSeek score 82.70

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: Genome
length_bp: 594
variable_sites: 264
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CTAACCGTGTTTCGACTCGT	TCGAAGTTATGGGCAACGAG	50.0	50.0	58.0	58.0	1618 bp	0.5	59.6
ndhG_p2	CGATACGAGTCGATCTGCTG	TCGAAGTTATGGGCAACGAG	55.0	50.0	58.1	58.0	1818 bp	0.5	59.5
ndhG_p3	CTAACCGTGTTTCGACTCGT	TGGTCGCATCCATTTCGAAT	50.0	45.0	58.0	58.0	1480 bp	0.5	59.4
ndhG_p4	CTAACCGTGTTTCGACTCGT	GCTATGTGTGATGGCAAGGA	50.0	50.0	58.0	58.0	1063 bp	0.5	59.3
ndhG_p5	CAACCCCCTCTCCACAAAAA	TCGAAGTTATGGGCAACGAG	50.0	50.0	57.9	58.0	1528 bp	0.5	59.3