Hotspot

ndhD

Genome · 114985-116493 · 1509 bp

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Pi 0.482

MarkerSeek score 81.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: Genome
length_bp: 1509
variable_sites: 647
pi: 0.482
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGGGGTCAATCTATCGGGTA	TTGCCCAACGGATTTCTTGA	55.0	45.0	58.0	58.0	2243 bp	0.5	59.7
ndhD_p2	GGGGGTCAATCTATCGGGTA	TGTACCCAATGCGTAAGAGC	55.0	50.0	58.0	58.0	2374 bp	0.5	59.7
ndhD_p3	GGGGGTCAATCTATCGGGTA	CCCACGGACGTATTGGAAAT	55.0	50.0	58.0	58.0	2350 bp	0.5	59.6
ndhD_p4	CCCTATTCCCCGGAACTTTG	TTGCCCAACGGATTTCTTGA	55.0	45.0	57.9	58.0	1973 bp	0.5	59.5
ndhD_p5	GAAGCGTGGGGATCTTACTG	TTGCCCAACGGATTTCTTGA	55.0	45.0	58.1	58.0	2410 bp	0.5	59.4