Hotspot

ccsA

Genome · 113567-114538 · 972 bp

Back to Marsilea

Pi 0.479

MarkerSeek score 81.40

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: Genome
length_bp: 972
variable_sites: 406
pi: 0.479
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	CTCAAGCATCTCGGTTCGAA	CAAAGTTCCGGGGAATAGGG	50.0	55.0	58.0	57.9	1641 bp	0.5	59.5
ccsA_p2	GCGGCATTTTCTCGTTATCG	CAAAGTTCCGGGGAATAGGG	50.0	55.0	58.0	57.9	1613 bp	0.5	59.5
ccsA_p3	CTCAAGCATCTCGGTTCGAA	ACTTTTTCGCTGACGTCACT	50.0	45.0	58.0	58.1	1692 bp	0.5	59.5
ccsA_p4	GCGGCATTTTCTCGTTATCG	ACTTTTTCGCTGACGTCACT	50.0	45.0	58.0	58.1	1664 bp	0.5	59.4
ccsA_p5	CTCAAGCATCTCGGTTCGAA	ACCTTACACCCCAAAACCAC	50.0	50.0	58.0	57.9	1440 bp	0.5	59.4