Hotspot

ccsA-ndhD

Genome · 114539-114984 · 446 bp

Back to Marsilea

Pi 0.524

MarkerSeek score 76.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: Genome
length_bp: 446
variable_sites: 187
pi: 0.524
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CTGAGCTATGCAACTCCGTT	TTGGCTTCTCTCGCATTACC	50.0	50.0	58.0	58.0	1349 bp	0.5	59.7
ccsA-ndhD_p2	CGAGTCGAGCAACAGATTCA	TTGGCTTCTCTCGCATTACC	50.0	50.0	58.0	58.0	1260 bp	0.5	59.7
ccsA-ndhD_p3	GGGGGTCAATCTATCGGGTA	TTGGCTTCTCTCGCATTACC	55.0	50.0	58.0	58.0	861 bp	0.5	59.6
ccsA-ndhD_p4	CTGAGCTATGCAACTCCGTT	TCGCATTACCAGGAATGAGC	50.0	50.0	58.0	58.0	1339 bp	0.5	59.5
ccsA-ndhD_p5	CGAGTCGAGCAACAGATTCA	TCGCATTACCAGGAATGAGC	50.0	50.0	58.0	58.0	1250 bp	0.5	59.5