Hotspot

trnL-UAG-ccsA

Genome · 113167-113566 · 400 bp

Back to Marsilea

Pi 0.547

MarkerSeek score 78.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: Genome
length_bp: 400
variable_sites: 198
pi: 0.547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TCAGCTTTTGAATGCCCACT	AGCGACCGTCATACCTTTTC	45.0	50.0	58.0	58.0	1272 bp	0.5	59.9
trnL-UAG-ccsA_p2	GTCTGGACTAGAAACCGCTG	AGCGACCGTCATACCTTTTC	55.0	50.0	58.0	58.0	1049 bp	0.5	59.9
trnL-UAG-ccsA_p3	CTCAAGCATCTCGGTTCGAA	AGCGACCGTCATACCTTTTC	50.0	50.0	58.0	58.0	575 bp	0.5	59.9
trnL-UAG-ccsA_p4	AAGTGAAGCGCATCATACGT	AGCGACCGTCATACCTTTTC	45.0	50.0	58.0	58.0	1168 bp	0.5	59.9
trnL-UAG-ccsA_p5	TCAGCTTTTGAATGCCCACT	TGAATCTGTTGCTCGACTCG	45.0	50.0	58.0	58.0	1669 bp	0.5	59.9