Hotspot

rpl32-trnP-GGG

Genome · 111784-112832 · 1049 bp

Back to Marsilea

Pi 0.505

MarkerSeek score 79.90

Primer available yes

Label rpl32-trnP-GGG

Feature Metadata

label_name: rpl32-trnP-GGG
feature_type: igs
region: Genome
length_bp: 1049
variable_sites: 424
pi: 0.505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnP-GGG_p1	ACCCCTGCTTTGGAAAGAAG	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	1871 bp	0.5	60.0
rpl32-trnP-GGG_p2	ACCCCTGCTTTGGAAAGAAG	CGATAACGAGAAAATGCCGC	50.0	50.0	58.0	58.0	1899 bp	0.5	59.9
rpl32-trnP-GGG_p3	ACTCATTTGTCACGGATCCG	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	1948 bp	0.5	59.9
rpl32-trnP-GGG_p4	ACTCATTTGTCACGGATCCG	CGATAACGAGAAAATGCCGC	50.0	50.0	58.0	58.0	1976 bp	0.5	59.9
rpl32-trnP-GGG_p5	CGAGGTCCGACGTTAACATT	TTCGAACCGAGATGCTTGAG	50.0	50.0	58.0	58.0	1991 bp	0.5	59.9