Hotspot

trnS-UGA-psbC

Genome · 34586-35470 · 885 bp

Back to Marsilea

Pi 0.0815

MarkerSeek score 80.50

Primer available yes

Label trnS-UGA-psbC

Feature Metadata

label_name: trnS-UGA-psbC
feature_type: igs
region: Genome
length_bp: 885
variable_sites: 71
pi: 0.0815
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbC_p1	ATGGTCGAGTGGTTTAAGGC	CGTTCTTTCAATGACTCCCCT	50.0	47.6	57.9	57.9	996-1045 bp	1.0	77.3
trnS-UGA-psbC_p2	GGGTTCGAATCCCTCTCTCT	CGTTCTTTCAATGACTCCCCT	55.0	47.6	58.2	57.9	936-985 bp	1.0	76.9
trnS-UGA-psbC_p3	CTTGAAAACTGGCATGGTGC	CGTTCTTTCAATGACTCCCCT	50.0	47.6	58.2	57.9	970-1019 bp	1.0	76.7
trnS-UGA-psbC_p4	TCAGGAGAGATGGTCGAGTG	CGTTCTTTCAATGACTCCCCT	55.0	47.6	58.2	57.9	1005-1054 bp	1.0	76.2
trnS-UGA-psbC_p5	ATGGTCGAGTGGTTTAAGGC	ACCCGTTCTTTCAATGACTCC	50.0	47.6	57.9	58.2	999-1048 bp	1.0	76.0