Hotspot

psbK-psbI

Genome · 9885-10520 · 636 bp

Back to Marsilea

Pi 0.0849

MarkerSeek score 82.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: Genome
length_bp: 636
variable_sites: 50
pi: 0.0849
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CAGGCCGCGGTAAGTTTC	CGTCCAGGATCGTTTGACAA	61.1	50.0	58.8	58.2	705-749 bp	1.0	65.2
psbK-psbI_p2	CAGGCCGCGGTAAGTTTC	TTTACGTCCAGGATTCCGTC	61.1	50.0	58.8	57.3	721-765 bp	1.0	61.5
psbK-psbI_p3	CAGGCCGCGGTAAGTTTC	CTTTACGTCCAGGATTCCGT	61.1	50.0	58.8	57.3	722-766 bp	1.0	61.4
psbK-psbI_p4	CAGGCCGCGGTAAGTTTC	TCTTTACGTCCAGGATTCCG	61.1	50.0	58.8	57.0	723-767 bp	1.0	59.1
psbK-psbI_p5	CAGGCCGCGGTAAGTTTC	GTCCAGGATCGTTTGACAAGA	61.1	47.6	58.8	58.0	704-748 bp	1.0	58.6