Hotspot

psaC

SSC · 119800-120045 · 246 bp

Back to Marshallia

Pi 0.00102

MarkerSeek score 22.70

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 1
pi: 0.00102
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 22.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	CTGAAGCCGCTATTGGACTT	TCTTTCCTCCCCACATGGAT	50.0	50.0	58.0	58.0	1168 bp	1.0	80.8
psaC_p2	CTGAAGCCGCTATTGGACTT	AAACCAACACCCAGAACTCC	50.0	50.0	58.0	57.9	1253 bp	1.0	80.3
psaC_p3	CTGAAGCCGCTATTGGACTT	GAGACGTTTCTTTCCTCCCC	50.0	55.0	58.0	57.9	1176 bp	1.0	80.1
psaC_p4	GCCGCTATTGGACTTGCTAT	TCTTTCCTCCCCACATGGAT	50.0	50.0	58.1	58.0	1163 bp	1.0	80.1
psaC_p5	GCCGCTATTGGACTTGCTAT	AAACCAACACCCAGAACTCC	50.0	50.0	58.1	57.9	1248 bp	1.0	79.6