Hotspot

ndhF

SSC · 125034-127259 · 2226 bp

Back to Marshallia

Pi 0.00111

MarkerSeek score 36.80

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2226
variable_sites: 9
pi: 0.00111
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CGAACGAAGTAAGATCCGGT	GCAGAATACCGTCACCCATT	50.0	50.0	57.4	58.0	2535-2584 bp	1.0	76.4
ndhF_p2	CGAACGAAGTAAGATCCGGT	GAACACGCTCGACAAAATGG	50.0	50.0	57.4	58.0	2501-2550 bp	1.0	76.3
ndhF_p3	CGAACGAAGTAAGATCCGGT	ACGCTCGACAAAATGGAAGA	50.0	45.0	57.4	57.8	2497-2546 bp	1.0	75.4
ndhF_p4	CGAACGAAGTAAGATCCGGT	AAGAAACTGAACACGCTCGA	50.0	45.0	57.4	57.8	2509-2558 bp	1.0	74.9
ndhF_p5	CGAACGAAGTAAGATCCGGT	CACGCTCGACAAAATGGAAG	50.0	50.0	57.4	57.8	2498-2547 bp	1.0	74.8