Hotspot

rpl32-ndhF

SSC · 124061-125033 · 973 bp

Back to Marshallia

Pi 0.00363

MarkerSeek score 55.90

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 973
variable_sites: 12
pi: 0.00363
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TGACAAAGCTTTTAACGCCG	CTTTTTGTTGCCGTCGGAAA	45.0	45.0	57.6	57.8	1170-1181 bp	1.0	76.5
rpl32-ndhF_p2	TGACAAAGCTTTTAACGCCG	AAGACTTTTTGTTGCCGTCG	45.0	45.0	57.6	57.5	1174-1185 bp	1.0	74.3
rpl32-ndhF_p3	TGACAAAGCTTTTAACGCCG	ACGAAGACTTTTTGTTGCCG	45.0	45.0	57.6	57.5	1177-1188 bp	1.0	74.3
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	CGAAGACTTTTTGTTGCCGT	45.0	45.0	57.6	57.5	1176-1187 bp	1.0	74.3
rpl32-ndhF_p5	CGCCGCATATCCTTTCCTTT	CTTTTTGTTGCCGTCGGAAA	50.0	45.0	58.7	57.8	1152-1163 bp	1.0	74.1