Hotspot

trnL-UAG-rpl32

SSC · 123072-123895 · 824 bp

Back to Marshallia

Pi 0.00392
MarkerSeek score 63.60
Primer available yes
Label trnL-UAG-rpl32

Feature Metadata

label_name
trnL-UAG-rpl32
feature_type
igs
region
SSC
length_bp
824
variable_sites
12
pi
0.00392
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-rpl32_p1 AGCGTGTCTACCGATTTCAC CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 913-946 bp 1.0 80.3
trnL-UAG-rpl32_p2 CAGCGTGTCTACCGATTTCA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 58.0 57.6 914-947 bp 1.0 80.3
trnL-UAG-rpl32_p3 TCCTAAGAGCAGCGTGTCTA CGGCGTTAAAAGCTTTGTCA 50.0 45.0 57.9 57.6 923-956 bp 1.0 79.3
trnL-UAG-rpl32_p4 CCTAAGAGCAGCGTGTCTAC CGGCGTTAAAAGCTTTGTCA 55.0 45.0 57.8 57.6 922-955 bp 1.0 78.8
trnL-UAG-rpl32_p5 CTAAGAGCAGCGTGTCTACC CGGCGTTAAAAGCTTTGTCA 55.0 45.0 57.8 57.6 921-954 bp 1.0 78.8