Hotspot

psbE-petL

LSC · 64465-65753 · 1289 bp

Back to Marshallia

Pi 0.00116

MarkerSeek score 63.10

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1289
variable_sites: 6
pi: 0.00116
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	AGAACGTTCTCCTGTGCTTC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1338-1360 bp	1.0	69.9
psbE-petL_p2	ACGAATAACCAACCCGCAAT	AGCCGTTAGTAGAAAACCGA	45.0	45.0	58.2	56.6	1424-1446 bp	1.0	69.7
psbE-petL_p3	TGCTGACGAATAACCAACCC	AGCCGTTAGTAGAAAACCGA	50.0	45.0	58.2	56.6	1429-1451 bp	1.0	69.6
psbE-petL_p4	CGAATAACCAACCCGCAATG	AGCCGTTAGTAGAAAACCGA	50.0	45.0	57.8	56.6	1423-1445 bp	1.0	69.6
psbE-petL_p5	GTTCTCCTGTGCTTCCAGAC	AGCCGTTAGTAGAAAACCGA	55.0	45.0	58.2	56.6	1333-1355 bp	1.0	69.6