Hotspot

trnS-UGA-psbZ

LSC · 35110-35445 · 336 bp

Back to Marshallia

Pi 0.00361

MarkerSeek score 39.30

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 336
variable_sites: 4
pi: 0.00361
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	456 bp	1.0	78.9
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	GACCAACCATCAGGAGAAGC	55.0	55.0	58.0	58.3	458 bp	1.0	78.5
trnS-UGA-psbZ_p3	TCAACCACTCAGCCATCTCT	CCAACCATCAGGAGAAGCAA	50.0	50.0	58.4	57.8	457 bp	1.0	76.3
trnS-UGA-psbZ_p4	TCAACCACTCAGCCATCTCT	GACCAACCATCAGGAGAAGC	50.0	55.0	58.4	58.3	459 bp	1.0	75.8
trnS-UGA-psbZ_p5	TTCAACCACTCAGCCATCTC	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.5	57.8	458 bp	1.0	75.2