Hotspot

atpH-atpF

LSC · 26510-26906 · 397 bp

Back to Marshallia

Pi 0.00651

MarkerSeek score 59.30

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 397
variable_sites: 7
pi: 0.00651
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	471-482 bp	1.0	81.4
atpH-atpF_p2	GCCTGGTTGTAGCATTAGCA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.2	57.9	472-483 bp	1.0	80.8
atpH-atpF_p3	GCCTGGTTGTAGCATTAGCA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.2	57.9	502-513 bp	1.0	80.7
atpH-atpF_p4	AGCGGAGGGAAAAATACGAG	TGGCCCAAAGAAACGAAAGA	50.0	45.0	57.7	57.9	539-550 bp	1.0	80.4
atpH-atpF_p5	AGCGGAGGGAAAAATACGAG	ATTAAACCCGAAACTCCCGG	50.0	50.0	57.7	57.9	570-581 bp	1.0	79.8