Hotspot

trnS-GCU-trnC-GCA

LSC · 8466-9219 · 754 bp

Back to Marshallia

Pi 0.00522

MarkerSeek score 58.30

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 11
pi: 0.00522
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	882-890 bp	1.0	77.4
trnS-GCU-trnC-GCA_p2	CTTTCGTCCACTCAGCCATC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	58.6	58.4	818-826 bp	1.0	73.6
trnS-GCU-trnC-GCA_p3	AACGGAAAGAGAGGGATTCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.3	58.4	883-891 bp	1.0	73.2
trnS-GCU-trnC-GCA_p4	ACGGAAAGAGAGGGATTCGA	ATTCGTTTGTTGATGAGGCG	50.0	45.0	58.2	57.1	924-932 bp	1.0	73.0
trnS-GCU-trnC-GCA_p5	ACGGAAAGAGAGGGATTCGA	CGGCACCCAGATTTGAACT	50.0	52.6	58.2	58.1	906-914 bp	1.0	71.8