Hotspot

ndhJ

Genome · 51233-51709 · 477 bp

Back to Marchantia

Pi 0.014

MarkerSeek score 31.40

Primer available yes

Label ndhJ

Feature Metadata

label_name: ndhJ
feature_type: gene
region: Genome
length_bp: 477
variable_sites: 10
pi: 0.014
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ_p1	AAATTGCCGGGATAGCTCAG	TACCTGGATGTCCGCCTAAA	50.0	50.0	58.0	58.1	1059-1060 bp	1.0	83.3
ndhJ_p2	AAATTGCCGGGATAGCTCAG	TTACCTGGATGTCCGCCTAA	50.0	50.0	58.0	58.1	1060-1061 bp	1.0	83.3
ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TACCTGGATGTCCGCCTAAA	50.0	50.0	58.1	58.1	1015-1016 bp	1.0	83.3
ndhJ_p4	CCTCGTGTCACCAGTTCAAA	TTACCTGGATGTCCGCCTAA	50.0	50.0	58.1	58.1	1016-1017 bp	1.0	83.3
ndhJ_p5	AAATTGCCGGGATAGCTCAG	TTTACCTGGATGTCCGCCTA	50.0	50.0	58.0	58.1	1062 bp	0.5	58.7