Hotspot

atpB-rbcL

Genome · 55847-56354 · 508 bp

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Pi 0.0299

MarkerSeek score 67.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: Genome
length_bp: 508
variable_sites: 23
pi: 0.0299
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GCAACATCTAATACGGGACCA	GCTTTGAATCCAACACCTGC	47.6	50.0	57.8	57.9	647-689 bp	1.0	73.3
atpB-rbcL_p2	GCAACATCTAATACGGGACCA	GTCTCCGTTTGTGGTGACAT	47.6	50.0	57.8	58.1	623-665 bp	1.0	73.0
atpB-rbcL_p3	AGGCAACATCTAATACGGGAC	GCTTTGAATCCAACACCTGC	47.6	50.0	57.5	57.9	649-691 bp	1.0	71.0
atpB-rbcL_p4	GGCAACATCTAATACGGGACC	GCTTTGAATCCAACACCTGC	52.4	50.0	58.5	57.9	648-690 bp	1.0	70.7
atpB-rbcL_p5	AGGCAACATCTAATACGGGAC	GTCTCCGTTTGTGGTGACAT	47.6	50.0	57.5	58.1	625-667 bp	1.0	70.7