Hotspot

MapoCt113-rps4

Genome · 48933-49424 · 492 bp

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Pi 0.0664

MarkerSeek score 79.80

Primer available yes

Label MapoCt113-rps4

Feature Metadata

label_name: MapoCt113-rps4
feature_type: igs
region: Genome
length_bp: 492
variable_sites: 49
pi: 0.0664
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
MapoCt113-rps4_p1	TTCGAATCCCTCTCTTTCCG	AATGAGTTGCTAGTTGTAGAA	50.0	33.3	57.0	52.5	470-554 bp	1.0	51.7
MapoCt113-rps4_p2	TTCGAATCCCTCTCTTTCCG	AAATGAGTTGCTAGTTGTAGA	50.0	33.3	57.0	52.5	471-555 bp	1.0	51.6
MapoCt113-rps4_p3	TTCGAATCCCTCTCTTTCCG	AAATGAGTTGCTAGTTGTAGAA	50.0	31.8	57.0	53.4	471-555 bp	1.0	51.6
MapoCt113-rps4_p4	GGGTTCGAATCCCTCTCTTT	AATGAGTTGCTAGTTGTAGAA	50.0	33.3	56.9	52.5	473-557 bp	1.0	51.6
MapoCt113-rps4_p5	TTCGAATCCCTCTCTTTCCG	TCAAATTATTGATCGTGAATGGA	50.0	30.4	57.0	54.4	507-591 bp	1.0	51.1