Hotspot

psbC-MapoCt110

Genome · 41286-41406 · 121 bp

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Pi 0.0482

MarkerSeek score 70.30

Primer available yes

Label psbC-MapoCt110

Feature Metadata

label_name: psbC-MapoCt110
feature_type: igs
region: Genome
length_bp: 121
variable_sites: 9
pi: 0.0482
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-MapoCt110_p1	GCTGCAGCTGGTTTTGAAAA	GTTTATGGCGTCGGTCTTGA	45.0	50.0	58.1	58.3	251-272 bp	1.0	84.0
psbC-MapoCt110_p2	GCTGCAGCTGGTTTTGAAAA	AGATGGCCGAGTGGTTTATG	45.0	50.0	58.1	57.7	264-285 bp	1.0	83.5
psbC-MapoCt110_p3	GCTGCAGCTGGTTTTGAAAA	ATAGGAGAGATGGCCGAGTG	45.0	55.0	58.1	58.4	271-292 bp	1.0	83.1
psbC-MapoCt110_p4	GCTGCAGCTGGTTTTGAAAA	AGAGATGGCCGAGTGGTTTA	45.0	50.0	58.1	58.4	266-287 bp	1.0	82.6
psbC-MapoCt110_p5	GCTGCAGCTGGTTTTGAAAA	TATGGCGTCGGTCTTGAAAA	45.0	45.0	58.1	57.5	248-269 bp	1.0	82.6