Hotspot

ndhB-MapoCt098

Genome · 3556-3678 · 123 bp

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Pi 0.084

MarkerSeek score 63.40

Primer available yes

Label ndhB-MapoCt098

Feature Metadata

label_name: ndhB-MapoCt098
feature_type: igs
region: Genome
length_bp: 123
variable_sites: 15
pi: 0.084
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-MapoCt098_p1	TTTGGTGTGGATGGCAATCA	CTCGCGTGTCTACCATTTCA	45.0	50.0	58.0	58.0	426-452 bp	1.0	88.6
ndhB-MapoCt098_p2	TTTGGTGTGGATGGCAATCA	GACTCGAACCTCCATGCTTT	45.0	50.0	58.0	57.9	464-490 bp	1.0	87.5
ndhB-MapoCt098_p3	TTTGGTGTGGATGGCAATCA	GCGTGTCTACCATTTCACCA	45.0	50.0	58.0	58.2	423-449 bp	1.0	87.1
ndhB-MapoCt098_p4	TTTGGTGTGGATGGCAATCA	TCTCGCGTGTCTACCATTTC	45.0	50.0	58.0	57.7	427-453 bp	1.0	86.5
ndhB-MapoCt098_p5	TTTGGTGTGGATGGCAATCA	TTGAATCTCGCGTGTCTACC	45.0	50.0	58.0	57.7	432-458 bp	1.0	86.4