Hotspot

ndhB-trnL-CAA

IRa · 146324-148491 · 2168 bp

Back to Marattia

Pi 0.0756
MarkerSeek score 72.30
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
2168
variable_sites
114
pi
0.0756
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.70
markerseek_score
72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 TGCAATCGCACAGAATACTT GCGTGTCTACCATTTCACCA 40.0 50.0 55.8 58.2 1674-2223 bp 1.0 69.2
ndhB-trnL-CAA_p2 TGCAATCGCACAGAATACTT TGCGTGTCTACCATTTCACC 40.0 50.0 55.8 58.2 1675-2224 bp 1.0 69.2
ndhB-trnL-CAA_p3 TGCAATCGCACAGAATACTTTC GCGTGTCTACCATTTCACCA 40.9 50.0 57.7 58.2 1674-2223 bp 1.0 68.4
ndhB-trnL-CAA_p4 TGCAATCGCACAGAATACTTTC TGCGTGTCTACCATTTCACC 40.9 50.0 57.7 58.2 1675-2224 bp 1.0 68.4
ndhB-trnL-CAA_p5 TGCAATCGCACAGAATACTTT GCGTGTCTACCATTTCACCA 38.1 50.0 56.5 58.2 1674-2223 bp 1.0 67.4