Hotspot

trnL-CAA-ndhB

IRb · 94151-96318 · 2168 bp

Back to Marattia

Pi 0.0756

MarkerSeek score 72.30

Primer available yes

Label trnL-CAA-ndhB

Feature Metadata

label_name: trnL-CAA-ndhB
feature_type: igs
region: IRb
length_bp: 2168
variable_sites: 114
pi: 0.0756
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.70
markerseek_score: 72.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ndhB_p1	GCGTGTCTACCATTTCACCA	TGCAATCGCACAGAATACTT	50.0	40.0	58.2	55.8	1674-2223 bp	1.0	69.2
trnL-CAA-ndhB_p2	TGCGTGTCTACCATTTCACC	TGCAATCGCACAGAATACTT	50.0	40.0	58.2	55.8	1675-2224 bp	1.0	69.2
trnL-CAA-ndhB_p3	GCGTGTCTACCATTTCACCA	TGCAATCGCACAGAATACTTTC	50.0	40.9	58.2	57.7	1674-2223 bp	1.0	68.4
trnL-CAA-ndhB_p4	TGCGTGTCTACCATTTCACC	TGCAATCGCACAGAATACTTTC	50.0	40.9	58.2	57.7	1675-2224 bp	1.0	68.4
trnL-CAA-ndhB_p5	GCGTGTCTACCATTTCACCA	TGCAATCGCACAGAATACTTT	50.0	38.1	58.2	56.5	1674-2223 bp	1.0	67.4