Hotspot

rps8-rpl14

LSC · 85874-86319 · 446 bp

Back to Marattia

Pi 0.123

MarkerSeek score 78.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 446
variable_sites: 49
pi: 0.123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 78.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	ACAAACTCGGAACATGGCAT	AGCACGAGCAGCTATGAAAA	45.0	45.0	58.1	57.9	1613-1634 bp	1.0	86.0
rps8-rpl14_p2	ACAAACTCGGAACATGGCAT	CTGTAGTGAAACCAGGTCGG	45.0	55.0	58.1	57.9	1668 bp	0.5	58.6
rps8-rpl14_p3	ACAAACTCGGAACATGGCAT	CACGTATGGGCTCTGGAAAA	45.0	50.0	58.1	57.9	1710 bp	0.5	58.4
rps8-rpl14_p4	CGAGCTTCCCGATCTGTTAC	CTGTAGTGAAACCAGGTCGG	55.0	55.0	58.2	57.9	1436 bp	0.5	58.1
rps8-rpl14_p5	ACAAACTCGGAACATGGCAT	GCGGTACCTAATATGGCGTT	45.0	50.0	58.1	57.8	1268 bp	0.5	58.0