Hotspot

clpP-psbB

LSC · 77328-77775 · 448 bp

Back to Marattia

Pi 0.0851

MarkerSeek score 78.70

Primer available yes

Label clpP-psbB

Feature Metadata

label_name: clpP-psbB
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 36
pi: 0.0851
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP-psbB_p1	CGCACTATACGTCAACCCAA	CGACGGTATGGACACGATAC	50.0	55.0	58.0	58.0	533-558 bp	1.0	89.2
clpP-psbB_p2	AGCCTGAATGCTTGAACCAA	CGACGGTATGGACACGATAC	45.0	55.0	58.0	58.0	712-737 bp	1.0	87.5
clpP-psbB_p3	CGCACTATACGTCAACCCAA	ATACCCATGTTCCAGGACCA	50.0	50.0	58.0	58.0	1004-1029 bp	1.0	85.9
clpP-psbB_p4	AGCCTGAATGCTTGAACCAA	ATACCCATGTTCCAGGACCA	45.0	50.0	58.0	58.0	1183-1208 bp	1.0	85.4
clpP-psbB_p5	TTCTCCTCCTTCAGAACCGT	ATACCCATGTTCCAGGACCA	50.0	50.0	58.0	58.0	1680-1702 bp	1.0	85.0