Hotspot

psbD-trnT-GGU

LSC · 42425-43793 · 1369 bp

Back to Marattia

Pi 0.0856
MarkerSeek score 80.30
Primer available yes
Label psbD-trnT-GGU

Feature Metadata

label_name
psbD-trnT-GGU
feature_type
igs
region
LSC
length_bp
1369
variable_sites
115
pi
0.0856
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbD-trnT-GGU_p1 CGAAACGGTCTCTCCTTAGC GGTTCAAATCCGATAAAGGGC 55.0 47.6 58.1 57.3 1460-1476 bp 1.0 73.2
psbD-trnT-GGU_p2 GGTCTCTCCTTAGCCAGTCA GGTTCAAATCCGATAAAGGGC 55.0 47.6 58.1 57.3 1454-1470 bp 1.0 72.8
psbD-trnT-GGU_p3 GAAACGGTCTCTCCTTAGCC GGTTCAAATCCGATAAAGGGC 55.0 47.6 57.7 57.3 1459-1475 bp 1.0 71.6
psbD-trnT-GGU_p4 AAACGGTCTCTCCTTAGCCA GGTTCAAATCCGATAAAGGGC 50.0 47.6 58.4 57.3 1458-1474 bp 1.0 71.1
psbD-trnT-GGU_p5 TCTCCTTAGCCAGTCATCCA GGTTCAAATCCGATAAAGGGC 50.0 47.6 57.4 57.3 1450-1466 bp 1.0 69.6