Hotspot

trnD-GUC-trnY-GUA

LSC · 38231-38275 · 45 bp

Back to Marattia

Pi 0.178

MarkerSeek score 78.20

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 45
variable_sites: 8
pi: 0.178
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GTTCGAGACCCGTCAATCC	ACTGTAAATCCGCTGGCAAT	57.9	45.0	58.0	57.9	117-130 bp	1.0	75.8
trnD-GUC-trnY-GUA_p2	GGTTCGAGACCCGTCAATC	ACTGTAAATCCGCTGGCAAT	57.9	45.0	58.0	57.9	118-131 bp	1.0	75.7
trnD-GUC-trnY-GUA_p3	AGTTCAATCGGTTAGAGCACC	ACTGTAAATCCGCTGGCAAT	47.6	45.0	58.0	57.9	162-175 bp	1.0	74.6
trnD-GUC-trnY-GUA_p4	GTTCGAGACCCGTCAATCC	CTCGAGTGGTTAATGGGGAC	57.9	55.0	58.0	57.7	139-152 bp	1.0	73.5
trnD-GUC-trnY-GUA_p5	AGTTCAATCGGTTAGAGCACC	CTCGAGTGGTTAATGGGGAC	47.6	55.0	58.0	57.7	184-197 bp	1.0	72.7