Hotspot

trnG-UCC-trnR-UCU

LSC · 18621-18819 · 199 bp

Back to Marattia

Pi 0.106

MarkerSeek score 79.20

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 199
variable_sites: 21
pi: 0.106
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	ACCCTTAGCCTTCCAAGCTA	TGTCCTATCCATTAGACGATGG	50.0	45.5	58.0	56.9	275-298 bp	1.0	60.8
trnG-UCC-trnR-UCU_p2	TAGTGATGCGGGTTCGATTC	TGTCCTATCCATTAGACGATGG	50.0	45.5	57.8	56.9	257-280 bp	1.0	59.7
trnG-UCC-trnR-UCU_p3	CTAGTGATGCGGGTTCGATT	TGTCCTATCCATTAGACGATGG	50.0	45.5	57.8	56.9	258-281 bp	1.0	59.6
trnG-UCC-trnR-UCU_p4	ACCCTTAGCCTTCCAAGCTA	ATGTCCTATCCATTAGACGATGG	50.0	43.5	58.0	57.3	276-299 bp	1.0	56.5
trnG-UCC-trnR-UCU_p5	CCCTTAGCCTTCCAAGCTAG	TGTCCTATCCATTAGACGATGG	55.0	45.5	57.4	56.9	274-297 bp	1.0	56.0