Hotspot

trnS-CGA-psbI

LSC · 16467-16690 · 224 bp

Back to Marattia

Pi 0.0981

MarkerSeek score 78.60

Primer available yes

Label trnS-CGA-psbI

Feature Metadata

label_name: trnS-CGA-psbI
feature_type: igs
region: LSC
length_bp: 224
variable_sites: 21
pi: 0.0981
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-CGA-psbI_p1	GGGTTCGAATCCCTCTTTCT	ACCACTGTATAAACAAACAGCT	50.0	36.4	56.9	55.9	272-282 bp	1.0	49.0
trnS-CGA-psbI_p2	GGGTTCGAATCCCTCTTTCTC	ACCACTGTATAAACAAACAGCT	52.4	36.4	58.1	55.9	272-282 bp	1.0	49.0
trnS-CGA-psbI_p3	GGTTCGAATCCCTCTTTCTCC	ACCACTGTATAAACAAACAGCT	52.4	36.4	58.1	55.9	271-281 bp	1.0	49.0
trnS-CGA-psbI_p4	GGGTTCGAATCCCTCTTTCT	ACCACTGTATAAACAAACAGCTT	50.0	34.8	56.9	56.6	272-282 bp	1.0	49.0
trnS-CGA-psbI_p5	GGGTTCGAATCCCTCTTTCT	AACCACTGTATAAACAAACAGCT	50.0	34.8	56.9	56.6	273-283 bp	1.0	48.9