Hotspot

psbK-trnS-CGA

LSC · 15962-16381 · 420 bp

Back to Marattia

Pi 0.0985

MarkerSeek score 77.90

Primer available yes

Label psbK-trnS-CGA

Feature Metadata

label_name: psbK-trnS-CGA
feature_type: igs
region: LSC
length_bp: 420
variable_sites: 40
pi: 0.0985
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnS-CGA_p1	CGTGGATGTGATGCCAGTTA	ATTCGAACCCTCGTTAACCG	50.0	50.0	58.0	58.0	571-582 bp	1.0	87.9
psbK-trnS-CGA_p2	ATGGCAAGCAGCTGTAAGTT	ATTCGAACCCTCGTTAACCG	45.0	50.0	58.1	58.0	520-531 bp	1.0	87.8
psbK-trnS-CGA_p3	CGTGGATGTGATGCCAGTTA	CTTTTTGGAGAGATGGCCGA	50.0	50.0	58.0	57.9	1069 bp	1.0	86.2
psbK-trnS-CGA_p4	CGTGGATGTGATGCCAGTTA	TTTTTGGAGAGATGGCCGAG	50.0	50.0	58.0	57.9	1068 bp	1.0	86.2
psbK-trnS-CGA_p5	ACCTCCGAATAACAGGACCA	ATTCGAACCCTCGTTAACCG	50.0	50.0	58.1	58.0	1033-1044 bp	1.0	85.3