Hotspot

trnH-GUG-psbA

LSC · 7457-7920 · 464 bp

Back to Marattia

Pi 0.134

MarkerSeek score 73.30

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 44
pi: 0.134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCAAGCTGAGCACAACAT	50.0	45.0	57.9	58.0	871-1000 bp	1.0	87.7
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	CTGACGGTATGCCTCTAGGA	50.0	55.0	57.9	58.0	921-1050 bp	1.0	87.7
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	GAGTGGGAACTTAGCTTCCG	50.0	55.0	57.9	58.0	1039-1168 bp	1.0	87.4
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	GCCCGTAGTAGGTATCTGGT	50.0	55.0	57.9	58.0	722 bp	0.5	58.9
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	TGGCCCGTAGTAGGTATCTG	50.0	55.0	57.9	58.0	724 bp	0.5	58.9