Hotspot

rpoA

LSC · 73211-74227 · 1017 bp

Back to Marathrum

Pi 0.0059

MarkerSeek score 48.90

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1017
variable_sites: 6
pi: 0.0059
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	GGTACTGTCGTAGCCCTTTG	ATACGTAGACGGGGTCGAAT	55.0	50.0	58.0	58.0	1994-2018 bp	1.0	81.5
rpoA_p2	GTACTGTCGTAGCCCTTTGG	ATACGTAGACGGGGTCGAAT	55.0	50.0	58.0	58.0	1993-2017 bp	1.0	81.5
rpoA_p3	TAATGGTTTCAGTACCCGCG	ATACGTAGACGGGGTCGAAT	50.0	50.0	58.0	58.0	2101-2125 bp	1.0	81.4
rpoA_p4	GTCGTAGCCCTTTGGTTAGG	ATACGTAGACGGGGTCGAAT	55.0	50.0	58.0	58.0	1988-2012 bp	1.0	81.4
rpoA_p5	ATTCTTGCTCGAGCTGGATG	ATACGTAGACGGGGTCGAAT	50.0	50.0	58.0	58.0	2473-2497 bp	1.0	80.8