Hotspot

petG-trnW-CCA

LSC · 62436-62556 · 121 bp

Back to Marathrum

Pi 0.0496

MarkerSeek score 74.80

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 121
variable_sites: 6
pi: 0.0496
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TAGACGCGGTGATCAATTGG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.1	58.0	202 bp	1.0	81.6
petG-trnW-CCA_p2	TAGACGCGGTGATCAATTGG	ACGCTCTTAGTTCAGTTCGG	50.0	50.0	58.1	57.7	223 bp	1.0	78.8
petG-trnW-CCA_p3	TAGACGCGGTGATCAATTGG	CGCTCTTAGTTCAGTTCGGT	50.0	50.0	58.1	57.7	222 bp	1.0	78.8
petG-trnW-CCA_p4	GCGGTGATCAATTGGACCTT	AGAACGTGGGTCTCCAAAAC	50.0	50.0	58.5	58.0	197 bp	1.0	77.9
petG-trnW-CCA_p5	TAGACGCGGTGATCAATTGG	TCTCCAAAACCCAATGTCGT	50.0	45.0	58.1	57.4	192 bp	1.0	76.8