Hotspot

trnG-UCC-trnS-GCU

LSC · 48231-48881 · 651 bp

Back to Marathrum

Pi 0.0246

MarkerSeek score 72.90

Primer available yes

Label trnG-UCC-trnS-GCU

Feature Metadata

label_name: trnG-UCC-trnS-GCU
feature_type: igs
region: LSC
length_bp: 651
variable_sites: 16
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnS-GCU_p1	TGAACCTCGAAAGATCCTCT	CAATCCGACGCTTTAGACCA	45.0	50.0	55.6	58.0	780-783 bp	1.0	62.3
trnG-UCC-trnS-GCU_p2	TGAACCTCGAAAGATCCTCT	AATCCGACGCTTTAGACCAC	45.0	50.0	55.6	58.0	779-782 bp	1.0	62.3
trnG-UCC-trnS-GCU_p3	TGAACCTCGAAAGATCCTCT	AGCAATCCGACGCTTTAGAC	45.0	50.0	55.6	58.4	782-785 bp	1.0	59.4
trnG-UCC-trnS-GCU_p4	TGAACCTCGAAAGATCCTCT	CGACGCTTTAGACCACTCAG	45.0	55.0	55.6	58.4	775-778 bp	1.0	59.3
trnG-UCC-trnS-GCU_p5	TGGTCAGAATATGAACCTCGA	CAATCCGACGCTTTAGACCA	42.9	50.0	56.2	58.0	791-794 bp	1.0	59.0